Testing the effect of temperature and polyethylene glycol on neocortical slice viability

Final Report: 10 May 2010
Waikato Medical Research Foundation

Aims

There were two main aims of this project: to quantify the effect of artificial cerebrospinal fluid (ACSF) temperature and polyethylene glycol on tissue slice viability/health; and to describe how electrophysiologically recorded slice activity changes as tissue health deteriorates. The purpose of the latter is to provide an objective means of assessing slice health, using electrophysiological parameters.


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